Protocols – University of Copenhagen

Welcome to EU-Alba > Protocols

Protocols

Protocol 1

Used for sorghum and pearl millet in Burkina Faso

Collection and processing of E. alba material
● Wild, whole plants of E. alba (root, stem, leaves, flowers) are collected in October-November at the flowering stage (1 plant will be sufficient to treat 1000 seeds).
● Plants are dried in the shade for at least 3 weeks. Brush of remaining soil from roots.
● Powderize the material using a wooden pestle and a large mortar
● Remove large particular matter and store the remaining powder dry and in the dark (up to 3 years) until use.

Preparation of extract (Two days before sowing):
For treatment of 2000 seeds:
●Mix 100 ml water and 2.5 gram dry powder (2.5 gram ~ 11 ml powder)
(Leave the mixture overnight).

Seed treatment (One day before sowing):
● Filter the mixture above through a piece of cloth or use it directly.
● Add 2000 seeds (ca 50 grams) to the mixture
● Allow the seeds to soak for 6 hours (Noon to sunset).
● Spread the seeds on a piece of cloth and allow the seeds to dry over night.

Sowing of seeds:
● Sow the seeds by hand
Sorghum: 4-6 seeds per hill.
Millet: 6-8 seeds per hill
After 3 weeks : thin hills to max three plants per hill.

Protocol 2

Used for sorghum and pearl millet in Tanzania

Collection and processing of E. alba material

● Cultivated, whole plants of E. alba (root, stem, leaves, flowers) are collected at the flowering stage (1 plant will be sufficient to treat 500 seeds).
● Plants are dried in the shade for at least 3 weeks. Brush of remaining soil from roots.
● Powderize the material using a wooden pestle and a large mortar
● Remove large particular matter and store the remaining powder dry and in the dark (up to 3 years) until use.

Preparation of extract (Two days before sowing):
For treatment of 2000 seeds:
● Mix 100 ml water and 5 gram dry powder (5 gram ~ 22 ml powder) (Leave the mixture overnight).

Seed treatment (One day before sowing):
Use the mixture above directly (filtering is not recommended):
● Add 2000 seeds (ca 50 grams) to the mixture
● Allow the seeds to soak for 8 hours.
● Spread the seeds on a piece of cloth and allow the seeds to dry overnight.

Sowing of seeds:
● Sow the seeds by hand
Sorghum: 4-6 seeds per hill.
Millet: 6-8 seeds per hill
After 3 weeks : thin hills to max three plants per hill.

Protocol 3

Treatment of Sorghum and Millet seeds with Balanites aegyptiaca extract.

Tested in Burkina Faso

Collection and processing of B. aegyptiaca material

● Barks of B. aegyptiaca are collected from wild trees. Barks (soft parts) are removed from the trunk of the tree with the help of a pick and air-dried for at least 7 days.  
●Powderize the material using a wooden pestle and a large mortar
●Remove large particular matter and store the remaining powder dry and in the dark (up to 3 years) until use.

Preparation of extract  (Two days before sowing):
For treatment of 2000 seeds: 

  • Mix 100 ml water and 2.5  gram dry powder (2.5 gram ~ 11 ml powder)
     (Leave the mixture overnight).

Seed treatment (One day before sowing):
Use the mixture above directly (filtering is not recommended):
●Add 2000 seeds (ca 50 grams) to the mixture
●Allow the seeds to soak for 8 hours.
●Spread the seeds on a piece of cloth and allow the seeds to dry overnight.

Sowing of seeds:
●Sow the seeds by hand
       Sorghum: 4-6 seeds per hill.
       Millet: 6-8 seeds per hill
After 3 weeks : thin hills to max three plants per hill.